Immunohistochemistry (IHC), Immunofluorescence (IF), & In Situ Hybridization (ISH)

GLP and Non-GLP IHC Staining

StageBio operates a GLP-compliant laboratory dedicated to specialty histopathology and immunohistochemistry services.  Some of our services include: immunohistochemical staining (IHC), immunofluorescent staining (IF), in situ hybridization (ISH), whole slide scanning, morphometric analyses and more.

Expansive & Diverse Biomarkers

StageBio Antibody Icon-01StageBio offers a wide and diverse variety of biomarkers. If there's something you're looking for and don't see, our investigational team would be happy to assist you in developing, validating, and optimizing your antibody. Please reach out to and we will be happy to assist you. View our latest list of biomarkers alphabetically by antibody or indication.



StageBio’s experienced team is proud to offer a broad range of routine and advanced IHC, ISH, and IF staining techniques for both frozen and formalin-fixed, paraffin-embedded (FFPE) tissues. Numerous routine antibodies across multiple species have been validated in-house, including:

  • Mouse
  • Rat
  • Dog
  • Pig
  • Non-human primate
  • Human

We offer single and multiplex chromogenic and immunofluorescent IHC staining for all of your protein localization and co-localization studies.

Scientific Experts

Stage Bio Website - IHC_Webinar Headshots_Dr Matt Buccellato

Dr Matt Buccellato


Senior Pathologist

Stage Bio Website - IHC_Webinar Headshots_Dr Thomas Lemarchand

Dr Thomas Lemarchand


Director of Digital and Quantitative Pathology

StageBio has the ability to perform traditional manual staining, and also employs automation.  Automated stainers are an excellent approach for research applications and facilitate increased throughput and consistent results for larger studies.

Our highly trained team of scientists can assist with your research goals by providing input regarding which IHC markers will be most effective for your studies. We are proficient with IHC method development and will optimize the antibodies that fit your research needs. Additionally, we are happy to consult with you regarding recommendations for tissue collection and fixation procedures that will lead to the best possible staining results.

Common Questions


Can StageBio pick up our study?

Yes - StageBio has a staff courier and can arrange to pick up your study materials no matter the size or location – including internationally.


How do I fix specimens for immunostaining?

Place your tissue(s) in 10% neutral buffered formalin for between 24 to 48 hours, then place in 70% ethyl alcohol.


How do I arrange to audit your facilities?

Access and complete the Contact Us form within this website to set up a facility inspection.

Contact Us

Contact us to discuss your IHC needs!



I have evaluated thousands of slides that StageBio has prepared. Their consistency from slide to slide is remarkable. It makes my job as a pathologist much easier when we contract our work to StageBio!”

Director of Pathology
Contract Research Organization

We've always found the quality of work and GLP reporting we receive from StageBio to be top notch. Good customer service is hard to come by, but that is one of the things that keeps us coming back.”

Assistant Director of Medical Devices
Medical Device CRO

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